Immunoprecipitation methods to identify S-glutathionylation in target proteins
نویسندگان
چکیده
منابع مشابه
Current Methods for the Identification of Sites of S-Glutathionylation and S-Nitrosylation in Proteins
Chemical modification of protein thiols has profound effects on the structure and catalytic activity of proteins. These post-translational modifications are often transient in nature, and therefore characterization of them can be elusive, especially in the case of S-nitrosothiols. Many of the methods discussed here involve a trapping mechanism where the modified thiol is selectively reduced and...
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S-glutathionylation, the covalent attachment of a glutathione (GSH) to the sulfur atom of cysteine, is a selective and reversible protein post-translational modification (PTM) that regulates protein activity, localization, and stability. Despite its implication in the regulation of protein functions and cell signaling, the substrate specificity of cysteine S-glutathionylation remains unknown. B...
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Nitric oxide (NO) is an endogenous, diffusible, transcellular messenger shown to affect regulatory and signaling pathways with impact on cell survival. Exposure to NO can impart direct post-translational modifications on target proteins such as nitration and/or nitrosylation. As an alternative, after interaction with oxygen, superoxide, glutathione, or certain metals, NO can lead to S-glutathio...
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ژورنال
عنوان ژورنال: MethodsX
سال: 2019
ISSN: 2215-0161
DOI: 10.1016/j.mex.2019.09.001